The ENO-30 system is a specialized HPLC for separating and detecting nitrite and nitrate in various biological fluids. The system makes use of a simple Griess reaction, in which nitrite reacts to form a light absorbing compound that can easily be detected.
Compared to a typical plate or tube based Griess assay, the ENO-30 greatly enhances sensitivity and reproducibility due to it’s innovation detection technology.
High throughput analysis of samples can be achieved by pairing the instrument with an HPLC autosampler. This is impossible for gas phase chemiluminescence detectors often used to detect nitric oxide.
Data collection, calibration, and analysis are handled by Eicom’s Envision software package.
In addition, high throughput analysis is achieved by pairing the instrument with an HPLC autosampler. This is impossible for gas phase chemiluminescent detectors sometimes used to detect nitric oxide. Data collection, calibration, and analysis are handled by Eicom’s Envision software package.
Sample Data of ENO-30 NOx Analyzer
The data shown here is a typical profile obtained from a human plasma sample using Eicom ENO-30, NOx Analyzer. The peaks correspond to 110 nM nitrite and 73 µM nitrate in 10 µl applied sample to the ENO-30.
Perfect for Biological Samples
Sensitive enough for a small volume of biological samples. The typical sample is just 1-50 μL of blood plasma, urine, saliva, breath condensate, tissue homogenate, cell culture supernatant, or microdialysate. Simple sample preparation consists of the addition of an equal volume of methanol to inactive and precipitate proteins, whereas low protein samples can be direct-injected without prior manipulation.
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